Analytical methods

ExperimentID EXA000014
ChemicalID CH0000266
Chemical Name Caffeine
Official Method No
Chromatography LC
Chromatography mfr. Nexera XR, Shimadzu; or others
Column [i] Atlantis T3 100 m x 2.1 mm, 3 μm; [ii] L-column2 ODS 100 m x 2.1 mm, 3 μm
Column mfr. [i] Waters; [ii] CERI
Detector MS/MS
Detector mfr. QTRAP5500, AB Sciex; or others
Ionization SRM-ESI-positive
Extraction SPE
Extraction Details Oasis HLB Plus 225 mg; Eluted with Methanol
Clean-up 1st
Clean-up 1st Details
Clean-up 2nd
Clean-up 2nd Details
Clean-up Spike Caffeine-13C3
Syringe Spike
Derivatization
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 1.1 ng/L
Method Quantification Limit
Repeatability
Standard
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Protocol
Remarks
RegisterDate 2021/3/16
UpdateDate 2021/3/16