Analytical methods

ExperimentID EXA000209
ChemicalID CH0000939
Chemical Name hepta-PCNs
Official Method No
Chromatography GC
Chromatography mfr. 6890, Agilent; 7683, Agilent
Column DB-5ms 60 m x 0.32 mm
Column mfr. Agilent
Detector MS
Detector mfr. AutoSpec Ultima, Waters; AutoSpec Premier, Waters
Ionization SIM-EI
Extraction Solvent Extraction
Extraction Details Ultrasonic extraction: Extracted with Acetone. After filtration, wash the residue in Acetone.; Soxhlet Extraction: Extracted with Toluene
Clean-up 1st Sulfate treatment
Clean-up 1st Details Sulfate
Clean-up 2nd Malti-layer column cleanup; GPC
Clean-up 2nd Details Malti-layer column cleanup: Silica Gel, Copper powder, Silica Gel Sulfate, Anhydrous sodium sulfate. Washed/Eluted with Dichloromethane/Hexane (20:80); Gel permeation chromatography: Acetone/Cyclohexane (20:80), Precolumn: Shodex EV-G AC, Column: Shodex EV-2000 AC
Clean-up Spike 13C101,2,3,4,5,6,7-Heptachloronaphthalene
Syringe Spike 13C12-PCB#9, #19, #70, #111, #155, #178 or #202
Derivatization
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 0.2 pg/g dry
Method Quantification Limit 0.5 pg/g dry
Repeatability
Standard
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Protocol https://www.env.go.jp/chemi/kurohon/2019/shosai/3_7.pdf
Remarks Analysis method: p.438; Resolution: 10,000
RegisterDate 2021/5/24
UpdateDate 2021/5/24