Analytical methods

ExperimentID EXA000211
ChemicalID CH0000215
Chemical Name PCP
Official Method No
Chromatography GC
Chromatography mfr. 6890, Agilent; 7683, Agilent
Column DB-5ms 30 m x 0.25 mm, 0.25 μm
Column mfr. Agilent
Detector HRMS
Detector mfr. AutoSpec Ultima, Waters; AutoSpec Premier, Waters
Ionization SIM-EI
Extraction Solvent Extraction
Extraction Details Shaking extraction; Ultrasonic extraction: Extracted with Acetone
Clean-up 1st Washing
Clean-up 1st Details Washed in 5% Sodium chloride aqueous solution
Clean-up 2nd Column cleanup; Multilayer silica gel column cleanup
Clean-up 2nd Details Column cleanup: 5% Hidrous silica gel, Hexan (First fraction) and Dichloromethane (Second fraction); Only second fraction was ethylated.; Multilayer silica gel column cleanup: Silica gel sulfate, Silica gel, Silver nitrate silica gel, Anhydrous sodium sulfate; Washed in Hexan. Eluted with Hexane
Clean-up Spike 13C12-Pentachlorophenol
Syringe Spike 13C12-PCB 70
Derivatization Ethylation
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 6 pg/g dry
Method Quantification Limit 18 pg/g dry
Repeatability
Standard
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Protocol https://www.env.go.jp/chemi/kurohon/2019/shosai/3_7.pdf
Remarks Analysis method: p.451; Resolution: 10,000
RegisterDate 2021/5/24
UpdateDate 2021/5/24