| ExperimentID |
EXA000298 |
| ChemicalID |
CH0001618
|
| Chemical Name |
Cyanamide |
| Official Method |
No |
| Chromatography |
LC |
| Chromatography mfr. |
Prominence, Shimadzu |
| Column |
InertSustain C18 150mm x 2.1mm, 5μm |
| Column mfr. |
GL Sciences |
| Detector |
MS/MS |
| Detector mfr. |
3200 QTRAP, AB Sciex |
| Ionization |
SRM-ESI-negative |
| Extraction |
LLE; SPE |
| Extraction Details |
Extracted with Dichloromethane 3mL, Shaken then kept still standing; SPE extraction with Sep-Pak Silka Plus Long Cartridge 690 mg; Eluted with Dichloromethane/Acetonitrile (60:40) 10mL |
| Clean-up 1st |
Filtration |
| Clean-up 1st Details |
Filtered with GF/C filter 47mm, Whatman |
| Clean-up 2nd |
Washing |
| Clean-up 2nd Details |
Cartridge: Washed in Dichloromethane twice |
| Clean-up Spike |
|
| Syringe Spike |
|
| Derivatization |
10% Dansyl Chloride Acetone solution 60mg/mL and 4-Dimethylaminopyridine Acetone solution 200μL; Shaken and kept at 50 ℃ for 60 min. |
| Recovery Correction |
|
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
280 ng/L |
| Method Quantification Limit |
|
| Repeatability |
|
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
|
| Recovery (IS) |
|
| Protocol |
https://www.env.go.jp/chemi/kurohon/2020/shosai/5_1_2.pdf |
| Remarks |
Analysis method: p.3 |
| RegisterDate |
2022/1/25 |
| UpdateDate |
2022/1/25 |
