| ExperimentID |
EXA000299 |
| ChemicalID |
CH0001620
|
| Chemical Name |
Pyridostigmine |
| Official Method |
No |
| Chromatography |
LC |
| Chromatography mfr. |
Necera XR, Shimadzu; LC-20A, Shimadzu |
| Column |
Xbridge Amide 150mm x 2.1mm, 3.5μm |
| Column mfr. |
Waters |
| Detector |
MS/MS |
| Detector mfr. |
QTRAP 4500QT, Sciex; QTRAP4500, AB Sciex |
| Ionization |
SRM-ESI-positive |
| Extraction |
SPE |
| Extraction Details |
ENVI-Carb Plus 10mL/min.; Eluted with Formic acid/Methanol (0.4:99.6) 5mL |
| Clean-up 1st |
Washing |
| Clean-up 1st Details |
Cartridge: Washed with purified water |
| Clean-up 2nd |
|
| Clean-up 2nd Details |
|
| Clean-up Spike |
Pyridostigmine-d6 |
| Syringe Spike |
|
| Derivatization |
|
| Recovery Correction |
|
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
0.043 ng/L |
| Method Quantification Limit |
|
| Repeatability |
|
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
|
| Recovery (IS) |
|
| Protocol |
https://www.env.go.jp/chemi/kurohon/2020/shosai/5_1_2.pdf |
| Remarks |
Analysis method: p.4 |
| RegisterDate |
2022/1/25 |
| UpdateDate |
2022/1/25 |
