Analytical methods

ExperimentID EXA000309
ChemicalID CH0001627
Chemical Name Valproic acid
Official Method No
Chromatography GC
Chromatography mfr. TRACE GU Ultra, Thermo Fisher Scientific; 7890B, Agilent
Column J&W DB-5ms 30m x 0.25mm, 0.25μm
Column mfr. Agilent
Detector MS
Detector mfr. TSQ Quantum, Thermo Fisher Scientific; JMS-Q1500, JEOL
Ionization SIM-EI
Extraction SPE
Extraction Details pH adjustment: pH 3; Sep-Pak Plus PS-2 10mL/min.; Eluted with Dichloromethane
Clean-up 1st Washing
Clean-up 1st Details Cartridge: Washed with purified water
Clean-up 2nd Column cleanup
Clean-up 2nd Details Supelclean LC-Si; Dichloromethane/Hexane (30:70) 10mL; Eluted with Dichloromethane/hexane (70:30) 10mL
Clean-up Spike
Syringe Spike Naphthalene-d8
Derivatization N-Methyl-N-tert-(butyldimethylsilyl)trifluoroacetamide
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 3.1 ng/L
Method Quantification Limit
Repeatability
Standard
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Protocol https://www.env.go.jp/chemi/kurohon/2020/shosai/5_1_2.pdf
Remarks Analysis method: p.8
RegisterDate 2022/1/25
UpdateDate 2022/1/25