| ExperimentID |
EXA000316 |
| ChemicalID |
CH0000344
|
| Chemical Name |
Levofloxacin |
| Official Method |
No |
| Chromatography |
LC |
| Chromatography mfr. |
Nexera X2, Shimadzu; Necera XR, Shimadzu |
| Column |
Luxi-Cellulose-5 EC-C18 150mm x 2.0mm, 3μm |
| Column mfr. |
Phenomenex |
| Detector |
MS/MS |
| Detector mfr. |
QTRAP4500, AB Sciex; QTRAP 4500QT, Sciex |
| Ionization |
SRM-ESI-positive |
| Extraction |
SPE |
| Extraction Details |
pH adjustment: pH 10; Oasis HLB Plus LP 225g; Eluted with Methanol |
| Clean-up 1st |
Filtration; washing |
| Clean-up 1st Details |
Filtered with Hydrophilic PTFE Membrane filter H100A047A, Advantec; Cartridge: Washed with purified water |
| Clean-up 2nd |
Column cleanup |
| Clean-up 2nd Details |
Oasis MCX Plus 225 mg; Eluted with 1mol/L Ammonia methanol solution |
| Clean-up Spike |
Levofloxacin-d3; Levofloxacin-d8 used for some cases |
| Syringe Spike |
|
| Derivatization |
|
| Recovery Correction |
|
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
0.44 ng/L |
| Method Quantification Limit |
|
| Repeatability |
|
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
|
| Recovery (IS) |
|
| Protocol |
https://www.env.go.jp/chemi/kurohon/2020/shosai/5_1_2.pdf |
| Remarks |
Analysis method: p.10 |
| RegisterDate |
2022/1/25 |
| UpdateDate |
2022/1/25 |
