Analytical methods

ExperimentID EXA000341
ChemicalID CH0000049
Chemical Name PCB81
Official Method No
Chromatography GC
Chromatography mfr. 6890, Agilent; 7683, Agilent
Column HT8-PCB 60 m x 0.25 mm
Column mfr. GL Sciences
Detector HRMS
Detector mfr. AutoSpec Ultima, Waters; AutoSpec Premier, Waters
Ionization SIM-EI
Extraction SPE
Extraction Details Sequentially extracted with glass fiber filter GC50 and with SPE disk C18FF; Ultrasonic extraction with Acetone;
Clean-up 1st Multilayer column cleanup; GPC
Clean-up 1st Details Multilayer column cleanup: Silica Gel, Silica Gel Sulfate(50:50), Anhydrous sodium sulfate; Eluted with Hexane (First fraction), Dichloromethane/Hexane (20:80) (Second fraction)
Clean-up 2nd Column cleanup
Clean-up 2nd Details Florisil; Eluted with Dichloromethane/Hexane (20:80)
Clean-up Spike 13C12-PCB#3, #8, #11, #28, #31, #52, #77, #81, #101, #105, #114, #118, #123, #126, #138, #153, #156, #157, #167, #169, #170, #180, #189, #206, or #209
Syringe Spike 13C12-PCB#9, #19, #70, #111, #155, #178, or #202
Derivatization
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 0.2 pg/L
Method Quantification Limit 0.6 pg/L
Repeatability
Standard
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Protocol https://www.env.go.jp/chemi/kurohon/2020/shosai/5_3_2.pdf
Remarks Analysis method: p.1; Resolution: 10,000
RegisterDate 2022/6/02
UpdateDate 2022/6/02