| ExperimentID |
EXA000426 |
| ChemicalID |
CH0000824
|
| Chemical Name |
ΣPBDEs (Br4-Br10) |
| Official Method |
No |
| Chromatography |
GC |
| Chromatography mfr. |
6890, Agilent; 7683, Agilent |
| Column |
BPX-DXN 30 m x 0.25 mm or BP-1 15 m x 0.25 mm, 0.1 μm |
| Column mfr. |
Trajan; Trajan |
| Detector |
HRMS |
| Detector mfr. |
AutoSpec Ultima, Waters; AutoSpec Premier, Waters |
| Ionization |
SIM-EI |
| Extraction |
Solvent Extraction |
| Extraction Details |
Ultrasonic extraction: Extracted with Acetone. After filtration, wash the residue in Acetone.; Soxhlet Extraction: Extracted with Toluene. |
| Clean-up 1st |
Malti-layer column cleanup |
| Clean-up 1st Details |
Malti-layer column cleanup: Silica Gel, Copper powder, Silica Gel Sulfate, Anhydrous sodium sulfate; Eluted with Hexane (First fraction), Dichloromethane/Hexane (20:80) (Second fraction) |
| Clean-up 2nd |
GPC; Multi-layer column cleanup |
| Clean-up 2nd Details |
Gel permeation chromatography: Acetone/Cyclohexane (20:80), Precolumn: Shodex EV-G AC; Column: Shodex EV-2000 AC; Multi-layer column cleanup: Active carbon-dispersed silica gel, Sodium sulfate; Eluted with Dichloromethane/Hexane (25:75) |
| Clean-up Spike |
13C12-PBDE#3, #15, #28, #47, #99, #126, #153, #154, #183, #197, #207 or #209 |
| Syringe Spike |
PBDE#138; PBDE#206 |
| Derivatization |
|
| Recovery Correction |
|
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
13 pg/g dry |
| Method Quantification Limit |
30 pg/g dry |
| Repeatability |
|
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
|
| Recovery (IS) |
|
| Protocol |
https://www.env.go.jp/chemi/kurohon/2020/shosai/5_3_2.pdf |
| Remarks |
Analysis method: p2-3; Resolution: 10,000 |
| RegisterDate |
2022/7/12 |
| UpdateDate |
2022/7/12 |
