Analytical methods

ExperimentID EXA000451
ChemicalID CH0001175
Chemical Name Pentachloroanisole
Official Method No
Chromatography GC
Chromatography mfr. 6890, Agilent; 7683, Agilent
Column DB-5ms 30 m x 0.25 mm
Column mfr. Agilent
Detector HRMS
Detector mfr. AutoSpec Ultima, Waters; AutoSpec Premier, Waters
Ionization SIM-EI
Extraction Shaking extraction; Ultrasonic extraction
Extraction Details Extracted with Acetone
Clean-up 1st Washing
Clean-up 1st Details Washed in 5% Sodium chloride aqueous solution
Clean-up 2nd Column cleanup; Multilayer silica gel column cleanup
Clean-up 2nd Details Column cleanup: 5% Hidrous silica gel, Eluted with Hexane (First fraction) and Dichloromethane (Second fraction); Only second fraction was ethylated.; Multilayer column cleanup: Silica gel sulfate, Silica gel, Silver nitrate silica gel, Anhydrous sodium sulfate; Eluted with Hexane
Clean-up Spike 13C12-Pentachlorophenol
Syringe Spike 13C12-PCB#9, #19, #70, #111, #155, #178, or #202
Derivatization Ethylation
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 0.8 pg/g dry
Method Quantification Limit 2.1 pg/g dry
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Remarks Analysis method: p16; Resolution: 10,000
RegisterDate 2022/7/12
UpdateDate 2022/7/12