Analytical methods

ExperimentID EXA000506
ChemicalID CH0001648
Chemical Name Dechlorane 602
Official Method No
Chromatography GC
Chromatography mfr. 7890 series, Agilent
Column Rtx-1614 column (15 m x 0.25 mm x 0.1 μm
Column mfr. Restek
Detector MS/MS
Detector mfr. 7000 GC/MS Triple Quad, Agilent
Extraction ASE
Extraction Details Extracted with a mixture of Hexane and acetone (1:1, v/v) using a SE-100 High-Speed Solvent Extractor (Mitsubishi Chemical Analytech, Japan), running at 35 ℃, at 6 mL/min for 30 min.
Clean-up 1st Chromatography; SPE
Clean-up 1st Details 3 steps: [1] Gel-permeation chromatography; [2] SPE step using Supelclean TM sulfoxide cartoridge (3 g/6 mL, Sigma-Aldrich) conditioned with 20 mL hexane in order to remove vehicle oil-related matrices in the VP samples cartridge; [3] Activated silica chromatography; Eluted with 40 mL acetone/hexane mixture (1:9, v/v)
Clean-up 2nd Wash
Clean-up 2nd Details Washed with 5 mL hexane
Clean-up Spike 13C12-BDE#28, #47, #99, #100, #153, #154, #183, #197, #207, #209; 13C10-anti-DP
Syringe Spike
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 0.48-0.63 ng/g dry
Method Quantification Limit
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
RegisterDate 2022/8/08
UpdateDate 2022/8/08