| ExperimentID |
EXA000588 |
| ChemicalID |
CH0001652
|
| Chemical Name |
Ampicillin |
| Official Method |
No |
| Chromatography |
LC |
| Chromatography mfr. |
[1] LC-20A Series, Shimadzu; [2] LC-30A Series, Shimadzu |
| Column |
[i] YMC-Triart C18 150 mm x 3.1 mm, 3μm; [ii] Waters CORTECS C18 100 mm×2.1 mm, 1.6 μm |
| Column mfr. |
[i] YMC; [ii] Waters |
| Detector |
MS/MS |
| Detector mfr. |
[1] API4000 QTRAP, AB Sciex; [2] LCMS-8050, Shimadzu |
| Ionization |
SRM-ESI-positive |
| Extraction |
SPE |
| Extraction Details |
Oasis HLB Plus 225 mg; Eluted with Acetonitrile/purified water (90:10) |
| Clean-up 1st |
Washing |
| Clean-up 1st Details |
Washed in 0.1% formic acid |
| Clean-up 2nd |
|
| Clean-up 2nd Details |
|
| Clean-up Spike |
Ampicillin-d5 |
| Syringe Spike |
|
| Derivatization |
|
| Recovery Correction |
|
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
0.12 ng/L |
| Method Quantification Limit |
|
| Repeatability |
|
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
|
| Recovery (IS) |
|
| Protocol |
https://www.env.go.jp/chemi/kurohon/2021/shosai/5_1_2.pdf |
| Remarks |
Analysis method: p.1 |
| RegisterDate |
2022/10/17 |
| UpdateDate |
2022/10/17 |
