| ExperimentID |
EXA000627 |
| ChemicalID |
CH0001666
|
| Chemical Name |
[(3-Hexadecanamidopropyl)(dimethyl)ammonio]acetate |
| Official Method |
No |
| Chromatography |
LC |
| Chromatography mfr. |
1260, Agilent |
| Column |
Inertsil ODS-4 HP 150 mm x 2.1 mm, 3 μm |
| Column mfr. |
GL Sciences |
| Detector |
MS/MS |
| Detector mfr. |
QTRAP 4500QT, AB Sciex |
| Ionization |
SRM-ESI-positive |
| Extraction |
Solvent Extraction |
| Extraction Details |
Extracted with 28 % Ammonia water / Methanol (5:95) 30 mL, Shaken and kept still standing at 70 ℃ for 15 min. Then, centrifuged 3,000 rpm for 10 min. ( 20 min. in the 3rd round); Repeated these procedures three times |
| Clean-up 1st |
Column cleanup |
| Clean-up 1st Details |
Sep-Pak PS2 Plus Short Cartridge, 225 mg / 80 μm; Run the extract through the cartridge at 2 mL / min.; Remove interfering substance with purified water 2 mL, formic acid / purified water (2:98) 5 mL, Methanol 10 mL; Eluted with 28 % Ammonia water / Methanol (5:95) 10 mL. |
| Clean-up 2nd |
Column cleanup |
| Clean-up 2nd Details |
Oasis MCX Plus Short Cartridge 225 mg / 60 μm; Run the extract (through the former column cleanup) through the cartridge at 2 mL / min.; Remove interfering substance with purified water 5 mL; Eluted with Methanol 10 mL. |
| Clean-up Spike |
|
| Syringe Spike |
|
| Derivatization |
|
| Recovery Correction |
|
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
0.19 ng/g dry |
| Method Quantification Limit |
|
| Repeatability |
|
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
|
| Recovery (IS) |
|
| Protocol |
https://www.env.go.jp/chemi/kurohon/2021/shosai/5_2_2.pdf |
| Remarks |
Analysis method: p.2 |
| RegisterDate |
2022/12/08 |
| UpdateDate |
2022/12/08 |
