Analytical methods

ExperimentID EXA000628
ChemicalID CH0001667
Chemical Name [(3-Octadecanamidopropyl)(dimethyl)ammonio]acetate
Official Method No
Chromatography LC
Chromatography mfr. 1260, Agilent
Column Inertsil ODS-4 HP 150 mm x 2.1 mm, 3 μm
Column mfr. GL Sciences
Detector MS/MS
Detector mfr. QTRAP 4500QT, AB Sciex
Ionization SRM-ESI-positive
Extraction Solvent Extraction
Extraction Details Extracted with 28 % Ammonia water / Methanol (5:95) 30 mL, Shaken and kept still standing at 70 ℃ for 15 min. Then, centrifuged 3,000 rpm for 10 min. ( 20 min. in the 3rd round); Repeated these procedures three times
Clean-up 1st Column cleanup
Clean-up 1st Details Sep-Pak PS2 Plus Short Cartridge, 225 mg / 80 μm; Run the extract through the cartridge at 2 mL / min.; Remove interfering substance with purified water 2 mL, formic acid / purified water (2:98) 5 mL, Methanol 10 mL; Eluted with 28 % Ammonia water / Methanol (5:95) 10 mL.
Clean-up 2nd Column cleanup
Clean-up 2nd Details Oasis MCX Plus Short Cartridge 225 mg / 60 μm; Run the extract (through the former column cleanup) through the cartridge at 2 mL / min.; Remove interfering substance with purified water 5 mL; Eluted with Methanol 10 mL.
Clean-up Spike
Syringe Spike
Derivatization
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 0.095 ng/g dry
Method Quantification Limit
Repeatability
Standard
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Protocol https://www.env.go.jp/chemi/kurohon/2021/shosai/5_2_2.pdf
Remarks Analysis method: p.2
RegisterDate 2022/12/08
UpdateDate 2022/12/08