| ExperimentID |
EXA000631 |
| ChemicalID |
CH0001639
|
| Chemical Name |
N,N-Dimethyldithiocarbamamic acid |
| Official Method |
No |
| Chromatography |
LC |
| Chromatography mfr. |
ExionLC, AB Sciex |
| Column |
RP-Amide 100 mm x 2.1 mm, 3 μm |
| Column mfr. |
Ascentis |
| Detector |
MS/MS |
| Detector mfr. |
QTRAP 6500QT, AB Sciex |
| Ionization |
SRM-ESI-negative |
| Extraction |
Solvent Extraction |
| Extraction Details |
Extracted with Methanol 35 mL, 0.5 % Cysteine / EDTA 40 mL; Shaken vigorously for 15 min. Then, centrifuged 2,000 rpm for 10 min.; Repeated these procedures twice. |
| Clean-up 1st |
Column cleanup |
| Clean-up 1st Details |
Oasis HLB Plus, 225 mg / 60 μm; Run the extract (after derivatization) through the cartridge at 20 mL / min.; Remove interfering substance with purified water 5 mL; Eluted with Acetonitrile 4 mL |
| Clean-up 2nd |
|
| Clean-up 2nd Details |
|
| Clean-up Spike |
|
| Syringe Spike |
|
| Derivatization |
Dimethyl sulfate 100 μL; Adjusted to pH7.5-pH8.0 with sodium chloride; Shaken vigorously for 10 min. |
| Recovery Correction |
|
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
1.3 ng/g dry |
| Method Quantification Limit |
|
| Repeatability |
|
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
|
| Recovery (IS) |
|
| Protocol |
https://www.env.go.jp/chemi/kurohon/2021/shosai/5_2_2.pdf |
| Remarks |
Analysis method: p.4 |
| RegisterDate |
2022/12/08 |
| UpdateDate |
2022/12/08 |
