Analytical methods

ExperimentID EXA000660
ChemicalID CH0000306
Chemical Name Sulfamerazine
Official Method No
Chromatography HPLC
Chromatography mfr. Prominence UFLC XR, Shimadzu
Column Ascentis Express C18 analytical column (2.7 μm, 100 x 2.1 mm)
Column mfr. Supelco
Detector MS/MS
Detector mfr. AB SCIEX QTRAP 5500 mass spectrometer, Sciex
Ionization ESI
Extraction SPE
Extraction Details An aliquot (20 mL) of filtered water sample was spiked with internal standards and then loaded onto an Oasis HLB Plus Light cartridge. The analytes retained in the cartridge were eluted with methanol/MTBE (3 mL, 7:3 v/v), and the eluate was concentrated to 0.2 mL under N2 flow. The residue was reconstituted in acetonitrile/methanol/Milli-Q water (1 mL, 1:2:7 v/v/v). For analysis of compounds found at concentrations >500 ng/L in water samples, the sample was diluted 2-fold prior to instrumental analysis.
Clean-up 1st
Clean-up 1st Details
Clean-up 2nd
Clean-up 2nd Details
Clean-up Spike Sulfamethazine-d4
Syringe Spike
Recovery Correction 92 ± 4.8
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 0.0015 μg/L
Method Quantification Limit
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Remarks Sample: water
RegisterDate 2023/1/16
UpdateDate 2023/1/16