| ExperimentID |
EXA000696 |
| ChemicalID |
CH0000278
|
| Chemical Name |
Diltiazem |
| Official Method |
No |
| Chromatography |
HPLC |
| Chromatography mfr. |
Prominence UFLC XR, Shimadzu |
| Column |
Ascentis Express C18 analytical column (2.7 μm, 100 x 2.1 mm) |
| Column mfr. |
Supelco |
| Detector |
MS/MS |
| Detector mfr. |
AB SCIEX QTRAP 5500 mass spectrometer, Sciex |
| Ionization |
ESI |
| Extraction |
LLE |
| Extraction Details |
Plasma sample (200 μL) was spiked with internal standard solutions, and extracted with a mixture of acetate buffer solution pH 4 (100 μL) and acetonitrile/methanol (1:1 v/v) (600 μL). |
| Clean-up 1st |
SPE |
| Clean-up 1st Details |
Passed through a HybridSPE-Phospholipid cartridge to remove phospholipids and proteins. |
| Clean-up 2nd |
|
| Clean-up 2nd Details |
|
| Clean-up Spike |
Diltiazem-d3 |
| Syringe Spike |
|
| Derivatization |
|
| Recovery Correction |
97 ± 3.9 |
| Instrument Detection Limit |
|
| Instrument Quantification Limit |
|
| Method Detection Limit |
0.0048 ng/mL |
| Method Quantification Limit |
|
| Repeatability |
4.4 |
| Standard |
|
| Recovery (CRM) |
|
| Recovery (spiked) |
110 ± 4.8 |
| Recovery (IS) |
|
| Protocol |
|
| Remarks |
Sample: Fish plasma |
| RegisterDate |
2023/1/16 |
| UpdateDate |
2023/1/16 |
