Analytical methods

ExperimentID EXA000792
ChemicalID CH0001172
Chemical Name cis-Heptachlor epoxide
Official Method No
Chromatography GC
Chromatography mfr. 6890, Agilent; 7890, Agilent
Column RH-12ms 30 m x 0.25 mm
Column mfr. INVENTX
Detector HRMS
Detector mfr. AutoSpec Ultima, Waters; AutoSpec Premier, Waters
Ionization SIM-EI
Extraction Solvent Extraction
Extraction Details Extracted with Acetone; Shaken for 10 min., Sonicated for 15min., then Centrifugated (3,000rpm, 10 min.); Repeated these three times
Clean-up 1st Column cleanup
Clean-up 1st Details Florisil, Anhydrous sodium sulfate; Remove interfering substance with Hexane; Eluted with Dichloromethane/Hexane (20:80)
Clean-up 2nd GPC
Clean-up 2nd Details Gel permeation chromatography: Acetone/Cyclohexane (20:80), Precolumn: Shodex EV-G AC; Column: Shodex EV-2000 AC
Clean-up Spike 13C10-cis-heptachlor epoxide
Syringe Spike 13C12-PCB#9, #19, #70, #111, #155, #178, #202
Recovery Correction
Instrument Detection Limit
Instrument Quantification Limit
Method Detection Limit 0.7 pg/g dry
Method Quantification Limit 1.7 pg/g dry
Recovery (CRM)
Recovery (spiked)
Recovery (IS)
Remarks Analysis method: p.3-4; Resolution: 10,000
RegisterDate 2023/2/15
UpdateDate 2023/2/15